Mouse Epiregulin ELISA Kit (EREG)- 96 Tests

Detection method
Colorimetric
Sample type
Cell culture supernatant, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 10 pg/ml Range 8.23 pg/ml - 6000 pg/ml Recovery 83 % Sample specific recovery Sample type Average % Range Cell culture supernatant 102.6 94% - 110% Plasma 73.94 67% - 90% Assay duration Multiple steps standard assay Species reactivity Reacts with: Mouse Does not react with: Human Product overview Abcam’s Epiregulin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Epiregulin in plasma and cell culture supernatants. This assay employs an antibody specific for Mouse Epiregulin coated on a 96- well plate. Standards and samples are pipetted into the wells and Epiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Epiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Epiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. We have not been able to detect endogenous Mouse Epiregulin in normal serum with ab100682, only in serum spiked with Mouse Epiregulin. Platform Microplate