Mouse Fibronectin ELISA Kit- 96 Tests

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Key features and details
Sensitivity: 3 ng/ml
Range: 3.125 ng/ml – 200 ng/ml
Sample type: Cell culture supernatant, Plasma, Serum, Urine
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Mouse

EGP 19,152.00

Available on backorder

Sample type
Cell culture supernatant, Urine, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
= 3 ng/ml
Range
3.125 ng/ml – 200 ng/ml
Recovery
101 %

Assay time
3h 30m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat
Product overview
Mouse Fibronectin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab108849) is designed for the quantitative measurement of Fibronectin levels in plasma, serum, urine and cell culture supernatants.

A Fibronectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibronectin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibronectin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.

Platform
Microplate

SKU: ab108849-1x 96 test Category: Tag:

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