Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 6 pg/ml
Range
8.19 pg/ml - 2000 pg/ml
Recovery
> 100 %
Sample specific recovery
Sample type Average % Range
Cell culture supernatant 124.3 116% – 131%
Serum 111.8 101% – 120%
Plasma 99.08 90% – 107%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Mouse
Product overview
Abcam’s Galectin 3 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Galectin 3 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse Galectin 3 coated on a 96- well plate. Standards and samples are pipetted into the wells and Galectin 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Galectin 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Galectin 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Platform
Microplate
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