Mouse IL-17 ELISA Kit- 96 Tests
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Expiry Date
Key features and details
Sensitivity: 6 pg/ml
Range: 6.1 pg/ml – 600 pg/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Mouse
EGP 19,488.00
Available on backorder
Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 6 pg/ml
Range
6.1 pg/ml - 600 pg/ml
Recovery
99 %
Sample specific recovery
Sample type Average % Range
Cell culture supernatant 99.62 85% - 105%
Serum 101.38 87% - 107%
Plasma 97.47 84% - 103%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Mouse
Product overview
Abcam’s IL-17 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-17 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IL-17 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-17 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Platform
Microplate
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