Mouse IL-17B ELISA Kit- 96 Tests

Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 12 pg/ml Range 16.46 pg/ml - 12000 pg/ml Recovery > 100 %

Sample specific recovery
Sample type Average % Range
Cell culture supernatant 149.4 139% – 161%
Serum 118.9 104% – 128%
Plasma 129.3 92% – 155%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Mouse
Product overview
Abcam’s IL-17B Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-17B in serum (mouse IL-17B concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for mouse IL-17B coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17B present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-17B antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17B bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Platform
Microplate