Pig Prothrombin ELISA Kit- 96 Tests

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Key features and details
Sensitivity: 0.3 ng/ml
Range: 0.391 ng/ml – 400 ng/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Pig

EGP 19,152.00

Available on backorder

Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
= 0.3 ng/ml
Range
0.391 ng/ml – 400 ng/ml
Recovery
98 %

Assay time
4h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Pig
Product overview
Abcam’s Prothrombin Pig in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of prothrombin levels in plasma, serum and cell culture supernatants.

A Prothrombin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Prothrombin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Prothrombin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.

Platform
Microplate

SKU: ab108890-1x 96 test Category: Tag:

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