Rat ANP ELISA Kit (NPPA)- 96 Tests

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Key features and details
Sensitivity: 6.7 pg/ml
Range: 0.008 ng/ml – 2 ng/ml
Sample type: Cell culture supernatant, Plasma, Serum, Tissue
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Rat

EGP 19,152.00

Available on backorder

Sample type
Cell culture supernatant, Serum, Plasma, Tissue
Assay type
Sandwich (quantitative)
Sensitivity
= 6.7 pg/ml
Range
0.008 ng/ml – 2 ng/ml
Recovery
102 %

Assay time
5h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Rat
Predicted to work with: Pig
Product overview
Abcam’s ANP (NPPA) Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of ANP concentrations in cell culture supernatants, tissue-extract, serum and plasma.

An ANP specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an ANP specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of ANP captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.

Platform
Microplate

SKU: ab108797-1x 96 test Category: Tag:

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