Sample type
Cell culture supernatant, Urine, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
= 0.25 ng/ml
Range
0.391 ng/ml – 25 ng/ml
Recovery
95 %
Assay time
3h 30m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Rat
Product overview
Abcam’s C Reactive Protein (PTX1) Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C Reactive Protein in urine, plasma, serum, and cell culture supernatants.
A C Reactive Protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a C Reactive Protein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of C Reactive Protein captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.
Platform
Microplate
Properties
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