Rat CNTF ELISA Kit- 96 Tests

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Key features and details
Sensitivity: 10 pg/ml
Range: 8.23 pg/ml – 2000 pg/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Rat

EGP 19,488.00

Available on backorder

Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 10 pg/ml Range 8.23 pg/ml - 2000 pg/ml Recovery 100 % Sample specific recovery Sample type Average % Range Cell culture supernatant 97.3 89% - 108% Serum 105.6 93% - 112% Plasma 104.2 92% - 110% Assay duration Multiple steps standard assay Species reactivity Reacts with: Rat Product overview Abcam’s CNTF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat CNTF in serum, plasma and cell culture supernatants. This assay employs an antibody specific for rat CNTF coated on a 96-well plate. Standards and samples are pipetted into the wells and CNTF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat CNTF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CNTF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Platform Microplate

SKU: ab100758-1x 96 test Category: Tag:

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