Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 80 pg/ml
Range
68.59 pg/ml - 50000 pg/ml
Recovery
> 100 %
Sample specific recovery
Sample type Average % Range
Cell culture supernatant 115.5 104% – 124%
Serum 119.2 109% – 129%
Plasma 116.3 93% – 132%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Rat
Product overview
Abcam’s CXCL7 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CXCL7 in serum, plasma, cell culture supernatants.
This assay employs an antibody specific for Rat CXCL7 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CXCL7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Platform
Microplate
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