Rat IL-10 ELISA Kit- 96 Tests
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Expiry Date
Key features and details
Sensitivity: 45 pg/ml
Range: 0.063 ng/ml – 4 ng/ml
Sample type: Cell culture supernatant
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Rat
EGP 19,152.00
Available on backorder
Sample type
Cell culture supernatant
Assay type
Sandwich (quantitative)
Sensitivity
= 45 pg/ml
Range
0.063 ng/ml – 4 ng/ml
Assay time
4h 30m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Rat
Product overview
Abcam’s IL-10 Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-10 in cell culture supernatants.
An IL-10 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-10 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-10 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.
Platform
Microplate
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