Rat MCP1 ELISA Kit (CCL2)- 96 Tests

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Key features and details
Sensitivity: 15 pg/ml
Range: 24.67 pg/ml – 18000 pg/ml
Sample type: Cell culture extracts, Tissue Extracts
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Rat

EGP 19,488.00

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Detection method
Colorimetric
Sample type
Cell culture extracts, Tissue Extracts
Assay type
Sandwich (quantitative)
Sensitivity
< 15 pg/ml Range 24.67 pg/ml - 18000 pg/ml Recovery 93 % Sample specific recovery Sample type Average % Range Cell Lysate 93.73 84% - 105% Tissue Lysate 92.75 83% - 103% Assay duration Multiple steps standard assay Species reactivity Reacts with: Rat Product overview Abcam’s MCP1 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat MCP1 in cell lysates and tissue lysates. This assay employs an antibody specific for rat MCP1 coated on a 96-well plate. Standards and samples are pipette into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Notes Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances. It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. Platform Microplate

SKU: ab100778-1x 96 test Category: Tag:

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