Rat TNF alpha ELISA Kit- 96 Tests

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Key features and details
Sensitivity: 0.0082 ng/ml
Range: 0.0156 ng/ml – 1 ng/ml
Sample type: Cell culture supernatant
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Rat

EGP 19,152.00

Available on backorder

Sample type
Cell culture supernatant
Assay type
Sandwich (quantitative)
Sensitivity
= 0.0082 ng/ml
Range
0.016 ng/ml – 1 ng/ml
Assay time
4h 30m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Rat
Product overview
Abcam’s TNF alpha Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TNF alpha in rat cell culture supernatants.

A TNF alpha specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TNF alpha X specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TNF alpha captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.

Platform
Microplate

SKU: ab108913-1x 96 test Category: Tag:

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