Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 2 pg/ml
Range
0.82 pg/ml - 200 pg/ml
Recovery
97 %
Sample specific recovery
Sample type Average % Range
Cell culture supernatant 98.26 84% - 103%
Serum 97.43 83% - 103%
Plasma 96.42 85% - 104%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Rat
Product overview
Abcam’s VEGF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat VEGF in serum, plasma, cellculture supernatants.
This assay employs an antibody specific for rat VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Platform
Microplate
Elisa Kits
Rat VEGF ELISA Kit- 96 Tests
Availability:
Available on backorder
Expiry Date
Key features and details
Sensitivity: 2 pg/ml
Range: 0.82 pg/ml – 200 pg/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Rat
EGP 19,488.00
Available on backorder
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