Sample type
Urine, Serum, Hep Plasma, Cit plasma
Assay type
Competitive
Sensitivity
= 0.07 ng/ml
Range
0.2 ng/ml – 16 ng/ml
Recovery
= 105.5 %
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Mouse, Rat, Human
Product overview
Testosterone ELISA kit is a competitive ELISA designed for the accurate quantitative measurement of Testosterone in serum and plasma.
A 96-well plate has been precoated with anti-Testosterone antibodies. Samples and the Testosterone-HRP conjugate are added to the wells, where Testosterone in the sample competes with the added Testosterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Testosterone in the sample and the intensity is measured at 450 nm.
Platform
Microplate
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