Source Alteromonas macleodii 87
\nAssayed on Lambda DNA
\nUnit definition One unit of the enzyme is the amount required to hydrolyze 1 \u03bcg of Lambda DNA in 1 hour at 37\u00b0C in a total reaction volume of 50 \u03bcl.
\nOptimal SE-buffer W (10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA
\nOptimal temperature
\n37oC
\nStorage conditions 10 mM KH2PO4 (pH 7.2); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20\u00b0C.
\nLigation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
\nNon-specific hydrolisis No nonspecific activity was detected after incubation of 1 \u03bcg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37\u00b0C.
\nReagents Supplied with Enzyme 10 X SE-buffer W, BSA
\nMethylation sensitivity not tested
\nInactivation 20 minutes under
\n65oC
\nNotes To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 \u03bcg\/ml.
\nDo not use BSA for long incubation.<\/p>\n","protected":false},"excerpt":{"rendered":"
Recognition site
\nC\u2191YCGRG
\nGRGCY\u2193C<\/p>\n","protected":false},"featured_media":22065,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/22087"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=22087"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/22065"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=22087"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}