{"id":22612,"date":"2021-01-05T12:14:03","date_gmt":"2021-01-05T09:14:03","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/pnaclamp-nras-kit-25-test-kit\/"},"modified":"2021-01-05T14:16:41","modified_gmt":"2021-01-05T11:16:41","slug":"pnaclamp-nras-kit-25-test-kit","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/pnaclamp-nras-kit-25-test-kit\/","title":{"rendered":"PNAClamp\u2122 NRAS Kit 25 test\/kit"},"content":{"rendered":"
\n

Total 43 NRAS mutations can be detected.<\/span><\/p>\n

\u00a0<\/span><\/p>\n

<\/span><\/p>\n\n\n\n\n\n
\n

\u00a0Mutations<\/span><\/p>\n<\/td>\n

\n

Size\u00a0<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u00a0Codon 12<\/span><\/p>\n<\/td>\n

\n

Codon 13\u00a0<\/span><\/p>\n<\/td>\n

\n

Codon 59\u00a0<\/span><\/p>\n<\/td>\n

\n

Codon 61\u00a0<\/span><\/p>\n<\/td>\n

\n

Codon 117\u00a0<\/span><\/p>\n<\/td>\n

\n

Codon 146\u00a0<\/span><\/p>\n<\/td>\n

\n

25 tests\u00a0<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u00a09<\/span><\/p>\n<\/td>\n

\n

\u00a010<\/span><\/p>\n<\/td>\n

\n

\u00a03<\/span><\/p>\n<\/td>\n

\n

\u00a012<\/span><\/p>\n<\/td>\n

\n

\u00a04<\/span><\/p>\n<\/td>\n

\n

\u00a05<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/span><\/p>\n<\/div>\n

\n

FEATURES<\/p>\n

\n
    \n
  • 01<\/span>High sensitivity and specificity even with small amount of DNA (2% LOD with 10 ng wild\/mutant type mixed DNA)<\/span><\/li>\n
  • 02<\/span>Real-Time PCR based ready-to-use kit<\/span><\/li>\n
  • 03<\/span>Short running time (within 3 hours)<\/span><\/li>\n<\/ul>\n<\/div>\n<\/div>\n

    PROCEDURE<\/h4>\n
      \n
    1. Sample preparation<\/span><\/o:p><\/span><\/li>\n
    2. Extraction of DNA<\/span><\/o:p><\/span><\/li>\n
    3. Real-time PCR<\/span><\/o:p><\/span><\/li>\n
    4. Result Analysis<\/span>
      <\/li>\n<\/ol>\n

      COMPATIBLE SAMPE TYPE<\/h4>\n
      – FFPE tissue<\/div>\n

      COMPATIBLE REAL-TIME PCR MACHINE<\/h4>\n
        \n
      1. CFX96 <\/span>(Bio-Rad)<\/span>
        <\/li>\n
      2. ABI7500 <\/span>(Thermo Fisher Scientific)<\/span>
        <\/li>\n
      3. StepOnePlus <\/span>(Thermo Fisher Scientific)<\/span>
        <\/li>\n
      4. LightCycler 480II <\/span>(Roche)<\/span>
        <\/li>\n
      5. ABI7900HT <\/span>(Thermo Fisher Scientific)<\/span>
        <\/li>\n
      6. Rotor-Gene Q <\/span>(Qiagen)<\/span>
        <\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

        NRAS mutation is found in several cancers such as melanoma (13~25%), colorectal cancer (1~6%), lung cancer (1%), thyroid cancer (7%) and hepatocellular carcinoma (10%). It is know that the drug response against colorectal cancer medicine such as Erbitux and Cetuximab decreased and the prognosis of the metastatic colorectal cancer patient is bad if the patient has NRAS mutation.<\/p>\n","protected":false},"featured_media":22608,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/22612"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=22612"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/22608"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=22612"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}