{"id":22614,"date":"2021-01-05T12:14:04","date_gmt":"2021-01-05T09:14:04","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/pnaclamp-pik3ca-kit-25-test-kit\/"},"modified":"2021-01-05T13:42:54","modified_gmt":"2021-01-05T10:42:54","slug":"pnaclamp-pik3ca-kit-25-test-kit","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/pnaclamp-pik3ca-kit-25-test-kit\/","title":{"rendered":"PNAClamp\u2122 PIK3CA Kit 25 test\/kit"},"content":{"rendered":"
\n

Total 15 PIK3CA mutations can be detected.<\/span><\/p>\n

\u00a0<\/p>\n

<\/p>\n\n\n\n\n\n
\n

\u00a0Mutations<\/p>\n<\/td>\n

\n

\u00a0Size<\/p>\n<\/td>\n<\/tr>\n

\n

\u00a0Codon 542<\/p>\n<\/td>\n

\n

\u00a0Codon 545<\/p>\n<\/td>\n

\n

\u00a0Codon 546<\/p>\n<\/td>\n

\n

\u00a0Codon 1047<\/p>\n<\/td>\n

Codon 420<\/td>\n\n

\u00a025 tests<\/p>\n<\/td>\n<\/tr>\n

\n

\u00a03<\/p>\n<\/td>\n

\n

4\u00a0<\/p>\n<\/td>\n

\n

4\u00a0<\/p>\n<\/td>\n

\n

3\u00a0<\/p>\n<\/td>\n

\u00a01<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

<\/span><\/div>\n

\u00a0<\/p>\n<\/div>\n

\n

FEATURES<\/p>\n

\n
    \n
  • 01<\/span>High sensitivity and specificity even with small amount of DNA<\/span><\/li>\n
  • 02<\/span>Real-Time PCR based ready-to-use kit<\/span><\/li>\n
  • 03<\/span>Short running time (within 3 hours)<\/span><\/li>\n<\/ul>\n<\/div>\n<\/div>\n

    PROCEDURE<\/h4>\n
      \n
    1. Sample preparation<\/span><\/o:p><\/span><\/li>\n
    2. Extraction of DNA<\/span><\/o:p><\/span><\/li>\n
    3. Real-time PCR<\/span><\/o:p><\/span><\/li>\n
    4. Result Analysis<\/span><\/o:p><\/span><\/li>\n<\/ol>\n

      COMPATIBLE SAMPE TYPE<\/h4>\n
      – FFPE tissue
      – Plasma<\/div>\n

      COMPATIBLE REAL-TIME PCR MACHINE<\/h4>\n
        \n
      1. CFX96 <\/span>(Bio-Rad)<\/span>
        <\/li>\n
      2. ABI7500 <\/span>(Thermo Fisher Scientific)<\/span>
        <\/li>\n
      3. LightCycler 480II <\/span>(Roche)<\/span>
        <\/li>\n
      4. Rotor-Gene Q <\/span>(Qiagen)<\/span>
        <\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

        PIK3CA mutation activates PI3K (Phosphatidylinositol 3-kinase) protein and PI3K activates several signaling pathways of tyrosine kinase receptors. Activated tyrosine kinase represses the function and excitometabolism of protein which inhibits cell survival and division. These abnormal changes initiate the process of cancer development by activating the protein involved in cell growth. Point mutations in PIK3CA are detected with ca. 80% of frequency in exon 9 (interpreted as helical part of PI3K protein) and exon 20 (interpreted as kinase part), while other types of mutations are also seen in many different locations. PIK3CA mutations are found with 25 to 40 % frequency in various types of cancer like colorectal cancer, gastric cancer, lung cancer, brain cancer, endometrial cancer, ovarian cancer, breast cancer, and more.<\/p>\n

        PNAClamp\u2122 PIK3CA Mutation Detection Kit utilizes modified PCR technology, using optimized PNA probes that tightly bind to wild type DNA templates. This tight binding to the wild type DNA templates result in no amplification of the wild type DNA template in PCR reaction while the mutated genes, especially with SNPs, PCR amplification is processed for multiplication of the mutated DNA sequences.<\/p>\n","protected":false},"featured_media":22608,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/22614"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=22614"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/22608"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=22614"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}