{"id":22616,"date":"2021-01-05T12:14:05","date_gmt":"2021-01-05T09:14:05","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/pnaclamp-idh2-kit-25-test-kit\/"},"modified":"2021-01-05T13:32:03","modified_gmt":"2021-01-05T10:32:03","slug":"pnaclamp-idh2-kit-25-test-kit","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/pnaclamp-idh2-kit-25-test-kit\/","title":{"rendered":"PNAClamp\u2122 IDH2 Kit 25 test\/kit"},"content":{"rendered":"
\n

Total 11 IDH2 mutations can be detected.<\/span><\/p>\n

\n\n\n\n\n\n
\n

\u00a0Murations<\/span><\/p>\n<\/td>\n

\n

Size\u00a0<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u00a0Codon 140<\/span><\/p>\n<\/td>\n

\n

Codon 172\u00a0<\/span><\/p>\n<\/td>\n

\n

25 tests\u00a0<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u00a04<\/span><\/p>\n<\/td>\n

\n

7\u00a0<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\n

FEATURES<\/p>\n

\n

01<\/span>High sensitivity and specificity even with small amount of DNA (1% LOD with 10 ng wild\/mutant type mixed DNA)<\/span><\/p>\n

    \n
  • 02<\/span>Real-Time PCR based ready-to-use kit<\/span><\/li>\n
  • 03<\/span>Short running time (within 3 hours)<\/span><\/li>\n<\/ul>\n<\/div>\n<\/div>\n

    PROCEDURE<\/h4>\n
      \n
    1. Sample preparation<\/span><\/li>\n
    2. Extraction of DNA<\/span><\/li>\n
    3. Real-time PCR<\/span><\/li>\n
    4. Result Analysis<\/span><\/li>\n<\/ol>\n

      COMPATIBLE SAMPE TYPE<\/h4>\n
      – FFPE tissue
      – Biopsy tissue<\/div>\n
      COMPATIBLE REAL-TIME PCR MACHINE<\/div>\n<\/div>\n<\/div>\n
      \n
        \n
      1. CFX96<\/span>(Bio-Rad)<\/span><\/li>\n
      2. ABI7500 <\/span>(Thermo Fisher Scientific)<\/span><\/li>\n
      3. StepOnePlus <\/span>(Thermo Fisher Scientific)<\/span><\/li>\n
      4. Rotor-Gene Q <\/span>(Qiagen)<\/span><\/li>\n
      5. LightCycler 480II <\/span>(Roche)<\/span><\/li>\n
      6. ABI7900HT <\/span>(Thermo Fisher Scientific)<\/span><\/li>\n
      7. QuantStudio 5 <\/span>(Thermo Fisher Scientific)<\/span><\/li>\n<\/ol>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

        IDH2 is frequently mutated in glioma, acute myeloid leukemia, and myelodysplastic syndromes. Mutations generally involve point mutations at the R140 and R172 residues of the protein. Mutations in IDH1 and IDH2 result in deleterious \u201cgain of function\u201d: instead of converting isocitrate to \u03b1-ketoglutarate, mutated IDH1 or IDH2 converts isocitrate to 2-hydroxyglutarate. 2-hydroxyglutarate inhibits other proteins involved in epigenetic regulation.<\/p>\n","protected":false},"featured_media":22608,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/22616"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=22616"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/22608"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=22616"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}