{"id":27190,"date":"2021-02-14T18:26:57","date_gmt":"2021-02-14T15:26:57","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-dkk3-elisa-kit-dickkopf-3-96-tests\/"},"modified":"2021-02-14T23:32:24","modified_gmt":"2021-02-14T20:32:24","slug":"human-dkk3-elisa-kit-dickkopf-3-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-dkk3-elisa-kit-dickkopf-3-96-tests\/","title":{"rendered":"Human DKK3 ELISA Kit (Dickkopf-3)- 96 Tests"},"content":{"rendered":"<p>Product name<br \/>\nHuman DKK3 ELISA Kit (Dickkopf-3)<br \/>\nDetection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nCell culture supernatant, Serum, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n< 3 pg\/ml\nRange\n2.74 pg\/ml - 2000 pg\/ml\nRecovery\n> 85 %<\/p>\n<p>Sample specific recovery<br \/>\nSample type Average % Range<br \/>\nCell culture supernatant 96.23 85% &#8211; 105%<br \/>\nSerum 86.69 77% &#8211; 96%<br \/>\nPlasma 89.24 80% &#8211; 98%<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Human<br \/>\nProduct overview<br \/>\nAbcam\u2019s DKK3 (Dickkopf-3) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human DKK3 in serum, plasma, and cell culture supernatants.<\/p>\n<p>This assay employs an antibody specific for Human DKK3 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human DKK3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.<\/p>\n<p>Notes<br \/>\nOptimisation may be required with urine samples.<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 3 pg\/ml<br \/>\nRange: 2.74 pg\/ml &#8211; 2000 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27190"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27190"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27190"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}