Detection method
\nColorimetric
\nSample type
\nCell culture supernatant, Plasma
\nAssay type
\nSandwich (quantitative)
\nSensitivity
\n< 300 pg\/ml\nRange\n0.25 ng\/ml - 60 ng\/ml\nRecovery\n93 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 94.47 83% - 102%\nPlasma 93.54 83% - 103%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Human\nProduct overview\nAbcam\u2019s CX3CL1 (Fraktaline) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CX3CL1 in plasma and cell culture supernatants. (Human CX3CL1 concentration is pretty low in normal plasma, it may not be detected in this assay).\n\n\nThis assay employs an antibody specific for human CX3CL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CX3CL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CX3CL1 antibody is added. After washing away unbound biotinylated antibody, HRPconjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CX3CL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\n\nWe have not been able to detect endogenous Human Fractalkine in normal serum with ab100522, only in serum spiked with Human Fractalkine.\n\n\nGet higher sensitivity in only 90 minutes with Human Fractalkine ELISA Kit (CX3Cl1) (ab192145) from our SimpleStep ELISA\u00ae range.\n\nNotes\nOptimization may be required with urine samples\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"
Key features and details
\nSensitivity: 300 pg\/ml
\nRange: 0.25 ng\/ml – 60 ng\/ml
\nSample type: Cell culture supernatant, Plasma
\nDetection method: Colorimetric
\nAssay type: Sandwich (quantitative)
\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27202"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27202"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27202"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}