{"id":27208,"date":"2021-02-14T18:27:07","date_gmt":"2021-02-14T15:27:07","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-cxcl1-elisa-kit-gro-alpha-96-tests\/"},"modified":"2021-02-14T23:32:45","modified_gmt":"2021-02-14T20:32:45","slug":"human-cxcl1-elisa-kit-gro-alpha-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-cxcl1-elisa-kit-gro-alpha-96-tests\/","title":{"rendered":"Human CXCL1 ELISA Kit (GRO alpha)- 96 Tests"},"content":{"rendered":"<p>Detection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nCell culture supernatant, Serum, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n2 pg\/ml<br \/>\nRange<br \/>\n2 pg\/ml &#8211; 1000 pg\/ml<br \/>\nRecovery<br \/>\n> 99 %<\/p>\n<p>Sample specific recovery<br \/>\nSample type Average % Range<br \/>\nCell culture supernatant 112.2 101% &#8211; 122%<br \/>\nSerum 130.4 119% &#8211; 145%<br \/>\nPlasma 113.5 85% &#8211; 140%<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Human<br \/>\nProduct overview<br \/>\nAbcam\u2019s CXCL1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit (GRO alpha) is an in vitro enzyme designed for the quantitative measurement of Human GRO alpha in serum, plasma and cell culture supernatants.<\/p>\n<p>This assay employs an antibody specific for Human CXCL1 (GRO alpha) coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.<\/p>\n<p>Get higher sensitivity in only 90 minutes with Human CXCL1 ELISA Kit (GRO alpha) (ab190805) from our SimpleStep ELISA\u00ae range.<\/p>\n<p>Notes<br \/>\nOptimization may be required with urine samples<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 2 pg\/ml<br \/>\nRange: 2 pg\/ml &#8211; 1000 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27208"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27208"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27208"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}