Product name
\nHuman HB EGF ELISA Kit
\nDetection method
\nColorimetric
\nSample type
\nCell culture supernatant, Plasma
\nAssay type
\nSandwich (quantitative)
\nSensitivity
\n< 20 pg\/ml\nRange\n16.38 pg\/ml - 4000 pg\/ml\nRecovery\n90 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 81.22 73% - 90%\nPlasma 93.73 84% - 103%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Human\nProduct overview\nAbcam\u2019s HB EGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HB EGF in plasma and cell culture supernatants. (Human HB EGF concentration is pretty low in normal plasma, it may not be detected in this assay).\n\n\nThis assay employs an antibody specific for Human HB EGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HB EGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HB EGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HB EGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\n\nWe have not been able to detect the endogenous Human HB-EGF in normal serum with ab100531, only in serum spiked with Human HB-EGF.\n\nNotes\nOptimization may be required with urine samples\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"
Key features and details
\nSensitivity: 20 pg\/ml
\nRange: 16.38 pg\/ml – 4000 pg\/ml
\nSample type: Cell culture supernatant, Plasma
\nDetection method: Colorimetric
\nAssay type: Sandwich (quantitative)
\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27209"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27209"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27209"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}