{"id":27214,"date":"2021-02-14T18:27:18","date_gmt":"2021-02-14T15:27:18","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-interferon-gamma-elisa-kit-ifng-96-tests\/"},"modified":"2021-02-14T23:32:50","modified_gmt":"2021-02-14T20:32:50","slug":"human-interferon-gamma-elisa-kit-ifng-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-interferon-gamma-elisa-kit-ifng-96-tests\/","title":{"rendered":"Human Interferon gamma ELISA Kit (IFNG)- 96 Tests"},"content":{"rendered":"<p>Detection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nCell culture supernatant, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n< 15 pg\/ml\nRange\n20.6 pg\/ml - 15000 pg\/ml\nRecovery\n> 87 %<\/p>\n<p>Sample specific recovery<br \/>\nSample type Average % Range<br \/>\nCell culture supernatant 94.53 84% &#8211; 104%<br \/>\nPlasma 86.82 81% &#8211; 102%<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Human<br \/>\nProduct overview<br \/>\nAbcam\u2019s Interferon gamma (IFNG) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Interferon gamma- in plasma, and cell culture supernatants. (Human Interferon gamma concentration is pretty low in normal plasma, it may not be detected in this assay).<\/p>\n<p>This assay employs an antibody specific for Human Interferon gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and Interferon gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Interferon gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Interferon gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.<\/p>\n<p>We have not been able to detect endogenous Human Interferon gamma in normal serum with ab100537, only in serum spiked with Human Interferon gamma.<\/p>\n<p>Get results in 90 minutes with Human IFN gamma ELISA Kit (ab174443) from our SimpleStep ELISA\u00ae range.<\/p>\n<p>Notes<br \/>\nOptimization may be required with urine samples<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 15 pg\/ml<br \/>\nRange: 20.6 pg\/ml &#8211; 15000 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27214"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27214"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27214"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}