Detection method
\nColorimetric
\nSample type
\nSerum, Plasma
\nAssay type
\nSandwich (quantitative)
\nSensitivity
\n< 20 pg\/ml\nRange\n0.02 ng\/ml - 15 ng\/ml\nRecovery\n117 %\n\nSample specific recovery\nSample type Average % Range\nSerum 116.1 105% - 123%\nPlasma 118.1 99% - 126%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Human\nProduct overview\nHuman IgG ELISA kit is designed for the quantitative measurement of Human IgG in serum and plasma. (UniprotID: Q60590)\n\n\n \n\nNotes\nThe Human immune system consists of two functional components classified as the innate system (the physical, biochemical and cellular barriers), and the adaptive immune system (including lymphocytes and immunoglobulins). Immunoglobulins are the key elements of the humoral immune response in vertebrate against parasitic invasion.\n\nThe polypeptide chains of immunoglobulins composed of two identical heavy (H) chains and two identical light (L) chains linked together by inter-chain disulfide bonds. While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membranes. Based upon the variation of the constant region of the heavy chain, nine immunoglobulin heavy chain isotypes are found in humans: IgA (with subclasses IgA1 and IgA2), IgD, IgE, IgM, and IgG (with subclasses IgG1, IgG2, IgG3, and IgG4).\n\nIgG is the predominant immunoglobulin in the serum, which accounts for 75% of the total serum antibody of healthy individuals. IgG has a molecular weight of about 150 kDa. Four distinct subgroups of human IgG (IgG1, IgG2, IgG3, and IgG4) were first demonstrated in the 1960\u2019s by using polyclonal antisera prepared in animals immunized with human myeloma proteins.\n\nThe capture antibody of the kit ab100547 detects the Fc portion of the heavy chain of all subclasses of human IgG and the detection antibody detects the F(ab\u2019)2 fragment of IgG.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"
Key features and details
\nSensitivity: 20 pg\/ml
\nRange: 0.02 ng\/ml – 15 ng\/ml
\nSample type: Plasma, Serum
\nDetection method: Colorimetric
\nAssay type: Sandwich (quantitative)
\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27220"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27220"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27220"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}