{"id":27221,"date":"2021-02-14T18:27:23","date_gmt":"2021-02-14T15:27:23","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-igg1-elisa-kit-96-tests\/"},"modified":"2021-02-14T23:32:59","modified_gmt":"2021-02-14T20:32:59","slug":"human-igg1-elisa-kit-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-igg1-elisa-kit-96-tests\/","title":{"rendered":"Human IgG1 ELISA Kit- 96 Tests"},"content":{"rendered":"<p>Detection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nCell culture supernatant, Saliva, Urine, Serum, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n< 0.05 ng\/ml\nRange\n0.05 ng\/ml - 40 ng\/ml\nRecovery\n84 %\n\nSample specific recovery\nSample type Average % Range\nSerum 87 75% - 97%\nPlasma 81.5 71% - 94%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Human\nProduct overview\nAbcam\u2019s IgG1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IgG1 in serum and plasma.\n\nThis assay employs an antibody specific for Human IgG1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IgG1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IgG1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IgG1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\nNotes\nOptimization may be required with urine samples\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 0.05 ng\/ml<br \/>\nRange: 0.05 ng\/ml &#8211; 40 ng\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Saliva, Serum, Urine<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27221"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27221"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27221"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}