{"id":27222,"date":"2021-02-14T18:27:23","date_gmt":"2021-02-14T15:27:23","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-il-10-elisa-kit-96-tests-2\/"},"modified":"2021-02-14T23:33:00","modified_gmt":"2021-02-14T20:33:00","slug":"human-il-10-elisa-kit-96-tests-2","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-il-10-elisa-kit-96-tests-2\/","title":{"rendered":"Human IL-10 ELISA Kit- 96 Tests"},"content":{"rendered":"<p>Detection method<br \/>\nColorimetric<br \/>\nPrecision<br \/>\nIntra-assay<br \/>\nSample n Mean SD CV%<br \/>\nOverall    < 10%\nInter-assay\nSample n Mean SD CV%\nOverall    < 12%\nSample type\nCell culture supernatant, Serum, Plasma\nAssay type\nSandwich (quantitative)\nSensitivity\n< 1 pg\/ml\nRange\n2.34 pg\/ml - 150 pg\/ml\nRecovery\n100 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 98.37 87% - 110%\nSerum 101.94 87% - 108%\nPlasma 102.39 93% - 109%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Human\nProduct overview\nAbcam\u2019s IL-10 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-10 in serum, plasma and cell culture supernatants.\n\n\nThis assay employs an antibody specific for Human IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\n\nGet results in 90 minutes with Human IL-10 ELISA Kit (ab185986) from our SimpleStep ELISA\u00ae range.\n\n\n \n\nNotes\nOptimization may be required with urine samples\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 1 pg\/ml<br \/>\nRange: 2.34 pg\/ml &#8211; 150 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27222"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27222"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27222"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}