Detection method
\nColorimetric
\nSample type
\nCell culture supernatant, Serum, Plasma
\nAssay type
\nSandwich (quantitative)
\nSensitivity
\n< 3 pg\/ml\nRange\n3.29 pg\/ml - 800 pg\/ml\nRecovery\n98 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 101 89% - 108%\nSerum 97.89 85% - 104%\nPlasma 95.5 83% - 103%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Human\nProduct overview\nAbcam\u2019s IL-11 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-11 in serum, plasma, and cell culture supernatants.\n\n\nThis assay employs an antibody specific for Human IL-11 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-11 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-11 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-11 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\n\nGet higher sensitivity in only 90 minutes with Human IL-11 ELISA Kit (ab189569) from our SimpleStep ELISA\u00ae range.\n\nNotes\nOptimization may be required with urine samples\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"
Key features and details
\nSensitivity: 3 pg\/ml
\nRange: 3.29 pg\/ml – 800 pg\/ml
\nSample type: Cell culture supernatant, Plasma, Serum
\nDetection method: Colorimetric
\nAssay type: Sandwich (quantitative)
\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27224"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27224"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27224"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}