Detection method
\nColorimetric
\nSample type
\nCell culture supernatant, Plasma
\nAssay type
\nSandwich (quantitative)
\nSensitivity
\n< 1 pg\/ml\nRange\n0.8 pg\/ml - 600 pg\/ml\nRecovery\n88 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 95.52 88% - 109%\nPlasma 85.63 79% - 101%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Human\nProduct overview\nAbcam\u2019s IL-12 p70 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-12 p70 in plasma and cell culture supernatants.\n\n\n This assay employs an antibody specific for Human IL-12 p70 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-12 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 p70 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\n\nWe have not been able to detect endogenous Human IL-12 p70 in normal serum with ab100552, only in serum spiked with Human IL-12 p70.\n\n\nGet results in 90 minutes with Human IL-12p40\/p70 ELISA Kit (ab178009) from our SimpleStep ELISA\u00ae range.\n\nNotes\nOptimization may be required with urine samples\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"
Key features and details
\nSensitivity: 1 pg\/ml
\nRange: 0.8 pg\/ml – 600 pg\/ml
\nSample type: Cell culture supernatant, Plasma
\nDetection method: Colorimetric
\nAssay type: Sandwich (quantitative)
\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27225"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27225"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27225"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}