{"id":27235,"date":"2021-02-14T18:27:30","date_gmt":"2021-02-14T15:27:30","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-il-1-r4-elisa-kit-st2-96-tests\/"},"modified":"2021-02-14T23:33:11","modified_gmt":"2021-02-14T20:33:11","slug":"human-il-1-r4-elisa-kit-st2-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-il-1-r4-elisa-kit-st2-96-tests\/","title":{"rendered":"Human IL-1 R4 ELISA Kit (ST2)- 96 Tests"},"content":{"rendered":"<p>Product name<br \/>\nHuman IL-1 R4 ELISA Kit (ST2)<br \/>\nDetection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nCell culture supernatant, Serum, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n< 2 pg\/ml\nRange\n1.65 pg\/ml - 1200 pg\/ml\nRecovery\n97 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 98.43 91% - 105%\nSerum 109.6 98% - 117%\nPlasma 84.06 67% - 102%\nAssay time\n5h 00m\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Human\nProduct overview\nHuman IL-1 R4 ELISA Kit (ST2) (ab100563) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-1 R4 in serum, plasma, and cell culture supernatants.\n\n\nThis assay employs an antibody specific for Human IL-1 R4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 R4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-1 R4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 R4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\nNotes\nOptimization may be required with urine samples.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 2 pg\/ml<br \/>\nRange: 1.65 pg\/ml &#8211; 1200 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27235"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27235"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27235"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}