{"id":27241,"date":"2021-02-14T18:27:43","date_gmt":"2021-02-14T15:27:43","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-il-5-elisa-kit-96-tests-2\/"},"modified":"2021-02-14T23:33:16","modified_gmt":"2021-02-14T20:33:16","slug":"human-il-5-elisa-kit-96-tests-2","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-il-5-elisa-kit-96-tests-2\/","title":{"rendered":"Human IL-5 ELISA Kit- 96 Tests"},"content":{"rendered":"<p>Detection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nCell culture supernatant, Serum, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n< 5 pg\/ml\nRange\n2.74 pg\/ml - 2000 pg\/ml\nRecovery\n95 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 94.83 84% - 103%\nSerum 95.46 83% - 103%\nPlasma 96.32 84% - 104%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Human\nProduct overview\nAbcam\u2019s IL-5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-5 in serum, plasma, and cell culture supernatants.\n\n\nThis assay employs an antibody specific for Human IL-5 coated on a 96-well plate. Standards and samples are pipette into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\nNotes\nOptimization may be required with urine samples.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 5 pg\/ml<br \/>\nRange: 2.74 pg\/ml &#8211; 2000 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27241"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27241"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27241"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}