{"id":27257,"date":"2021-02-14T18:27:52","date_gmt":"2021-02-14T15:27:52","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-mip3b-elisa-kit-ccl19-96-tests\/"},"modified":"2021-02-14T23:33:36","modified_gmt":"2021-02-14T20:33:36","slug":"human-mip3b-elisa-kit-ccl19-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-mip3b-elisa-kit-ccl19-96-tests\/","title":{"rendered":"Human MIP3b ELISA Kit (CCL19)- 96 Tests"},"content":{"rendered":"<p>Detection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nCell culture supernatant, Serum, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n< 2 pg\/ml\nRange\n2.05 pg\/ml - 500 pg\/ml\nRecovery\n78 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 71.8 65% - 89%\nSerum 84.43 75% - 94%\nPlasma 79.88 71% - 90%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Human\nProduct overview\nAbcam\u2019s MIP3b Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP3b in serum, plasma, and cell culture supernatants.\n\nThis assay employs an antibody specific for Human MIP3b coated on a 96- well plate. Standards and samples are pipetted into the wells and MIP3b present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIP3b antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3b bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\nNotes\nOptimization may be required with urine samples.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 2 pg\/ml<br \/>\nRange: 2.05 pg\/ml &#8211; 500 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27257"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27257"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27257"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}