{"id":27259,"date":"2021-02-14T18:27:53","date_gmt":"2021-02-14T15:27:53","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-mmp13-elisa-kit-96-tests\/"},"modified":"2021-02-14T23:33:38","modified_gmt":"2021-02-14T20:33:38","slug":"human-mmp13-elisa-kit-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-mmp13-elisa-kit-96-tests\/","title":{"rendered":"Human MMP13 ELISA Kit- 96 Tests"},"content":{"rendered":"<p>Detection method<br \/>\nColorimetric<br \/>\nPrecision<br \/>\nIntra-assay<br \/>\nSample n Mean SD CV%<br \/>\nOverall    < 10%\nInter-assay\nSample n Mean SD CV%\nOverall    < 12%\nSample type\nCell culture supernatant, Plasma\nAssay type\nSandwich (quantitative)\nSensitivity\n< 6 pg\/ml\nRange\n8.23 pg\/ml - 6000 pg\/ml\nRecovery\n93 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 94.72 84% - 103%\nPlasma 93.13 83% - 103%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Human\nProduct overview\nAbcam\u2019s MMP13 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human MMP13 in plasma and cell culture supernatants.\n\n\nThis assay employs an antibody specific for Human MMP13 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\n\nThe kit detects total MMP-13 levels.\n\n\nWe have not been able to detect endogenous Human MMP13 in normal serum with ab100605, only in serum spiked with Human MMP13.\n\nNotes\nOptimisation may be required with urine samples.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 6 pg\/ml<br \/>\nRange: 8.23 pg\/ml &#8211; 6000 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27259"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27259"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27259"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}