Product name
\nHuman NRG1 beta 1 ELISA Kit
\nDetection method
\nColorimetric
\nSample type
\nCell culture supernatant, Plasma
\nAssay type
\nSandwich (quantitative)
\nSensitivity
\n< 50 pg\/ml\nRange\n82.3 pg\/ml - 20000 pg\/ml\nRecovery\n99 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 114.9 102% - 123%\nPlasma 86.02 79% - 94%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Human\nProduct overview\nAbcam\u2019s Human NRG1 beta 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NRG1 beta 1 in plasma, and cell culture supernatants. (Collect plasma using EDTA or citrate as an anticoagulant. Heparinized plasma is not recommended. Human NRG1 beta 1 concentration is pretty low in normal plasma, it may not be detected in this assay).\n\n\nThis assay employs an antibody specific for Human NRG1 beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and NRG1 beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NRG1 beta 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\n\nWe have not been able to detect endogenous Human NRG1 beta 1 in normal serum with ab100614, only in serum spiked with Human NRG1 beta 1.\n\n\nThe ab100614 kit recognises the human HRG beta 1 protein (isoform 6) and has not been tested with other isoforms.\n\nNotes\nOptimization may be required with urine samples\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"
Key features and details
\nSensitivity: 50 pg\/ml
\nRange: 82.3 pg\/ml – 20000 pg\/ml
\nSample type: Cell culture supernatant, Plasma
\nDetection method: Colorimetric
\nAssay type: Sandwich (quantitative)
\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27265"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27265"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27265"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}