{"id":27269,"date":"2021-02-14T18:28:08","date_gmt":"2021-02-14T15:28:08","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-p-cadherin-elisa-kit-96-tests\/"},"modified":"2021-02-14T23:33:50","modified_gmt":"2021-02-14T20:33:50","slug":"human-p-cadherin-elisa-kit-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-p-cadherin-elisa-kit-96-tests\/","title":{"rendered":"Human P cadherin ELISA Kit- 96 Tests"},"content":{"rendered":"<p>Product name<br \/>\nHuman P cadherin ELISA Kit<br \/>\nDetection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nCell culture supernatant, Serum, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n< 20 pg\/ml\nRange\n2.47 pg\/ml - 18000 pg\/ml\nRecovery\n> 100 %<\/p>\n<p>Sample specific recovery<br \/>\nSample type Average % Range<br \/>\nCell culture supernatant 129.1 117% &#8211; 138%<br \/>\nSerum 121.3 111% &#8211; 131%<br \/>\nPlasma 114.9 105% &#8211; 125%<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Human<br \/>\nProduct overview<br \/>\nAbcam\u2019s P cadherin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human P cadherin in serum, plasma and cell culture supernatants.<\/p>\n<p>This assay employs an antibody specific for Human P cadherin coated on a 96-well plate. Standards and samples are pipetted into the wells and P cadherin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human P cadherin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of P cadherin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.<\/p>\n<p>Notes<br \/>\nOptimization may be required with urine samples<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 20 pg\/ml<br \/>\nRange: 2.47 pg\/ml &#8211; 18000 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27269"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27269"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27269"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}