{"id":27274,"date":"2021-02-14T18:28:10","date_gmt":"2021-02-14T15:28:10","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-pf4-elisa-kit-cxcl4-96-tests\/"},"modified":"2021-02-14T23:33:54","modified_gmt":"2021-02-14T20:33:54","slug":"human-pf4-elisa-kit-cxcl4-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-pf4-elisa-kit-cxcl4-96-tests\/","title":{"rendered":"Human PF4 ELISA Kit (CXCL4)- 96 Tests"},"content":{"rendered":"<p>Detection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nCell culture supernatant, Serum, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n< 20 pg\/ml\nRange\n20.58 pg\/ml - 15000 pg\/ml\nRecovery\n100 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 108.2 101% - 116%\nSerum 122.4 110% - 130%\nPlasma 119.6 111% - 127%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Human\nProduct overview\nAbcam\u2019s PF4 (CXCL4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PF4 in serum, plasma, and cell culture supernatants.\n\n\nThis assay employs an antibody specific for Human PF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and PF4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PF4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\n\nGet results in 90 minutes with Human PF4 ELISA Kit (CXCL4) (ab189573) from our SimpleStep ELISA\u00ae range.\n\nNotes\nOptimization may be required with urine samples.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 20 pg\/ml<br \/>\nRange: 20.58 pg\/ml &#8211; 15000 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27274"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27274"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27274"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}