Product name
\nHuman SAA ELISA Kit (SAA1)
\nDetection method
\nColorimetric
\nSample type
\nCell culture supernatant, Serum, Plasma
\nAssay type
\nSandwich (quantitative)
\nSensitivity
\n< 0.5 ng\/ml\nRange\n0.41 ng\/ml - 300 ng\/ml\nRecovery\n100 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 93.75 86% - 102%\nSerum 114.8 105% - 124%\nPlasma 112.1 83% - 137%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Human\nProduct overview\nHuman SAA ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SAA in serum, plasma and cell culture supernatants.\n\n\nThis assay employs an antibody specific for Human Serum Amyloid A coated on a 96-well plate. Standards and samples are pipetted into the wells and Serum Amyloid A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Serum Amyloid A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Serum Amyloid A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\n\n \n\n\n \n\nNotes\nOptimization may be required with urine samples. ab100635 cross reacts with SAA2.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"
Key features and details
\nSensitivity: 0.5 ng\/ml
\nRange: 0.41 ng\/ml – 300 ng\/ml
\nSample type: Cell culture supernatant, Plasma, Serum
\nDetection method: Colorimetric
\nAssay type: Sandwich (quantitative)
\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27281"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27281"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27281"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}