{"id":27282,"date":"2021-02-14T18:28:15","date_gmt":"2021-02-14T15:28:15","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-sdf1-alpha-elisa-kit-96-tests\/"},"modified":"2021-02-14T23:34:01","modified_gmt":"2021-02-14T20:34:01","slug":"human-sdf1-alpha-elisa-kit-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-sdf1-alpha-elisa-kit-96-tests\/","title":{"rendered":"Human SDF1 alpha ELISA Kit- 96 Tests"},"content":{"rendered":"<p>Detection method<br \/>\nColorimetric<br \/>\nPrecision<br \/>\nIntra-assay<br \/>\nSample n Mean SD CV%<br \/>\nOverall    < 10%\nInter-assay\nSample n Mean SD CV%\nOverall    < 12%\nSample type\nCell culture supernatant, Plasma\nAssay type\nSandwich (quantitative)\nSensitivity\n< 80 pg\/ml\nRange\n93.8 pg\/ml - 6000 pg\/ml\nRecovery\n> 80 %<\/p>\n<p>Sample specific recovery<br \/>\nSample type Average % Range<br \/>\nCell culture supernatant 94.63 86% &#8211; 104%<br \/>\nPlasma 89.34 85% &#8211; 104%<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Human<br \/>\nProduct overview<br \/>\nHuman SDF1 alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab100637) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SDF1 alpha in plasma and cell culture supernatants. (Human SDF1 alpha concentration is pretty low in normal plasma, it may not be detected in this assay).<\/p>\n<p>This assay employs an antibody specific for Human SDF1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and SDF1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SDF1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SDF1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.<\/p>\n<p>We have not been able to detect the endogenous Human SDF-1 alpha in normal serum with ab100637, only in serum spiked with Human SDF-1 alpha.<\/p>\n<p>Notes<br \/>\nOptimization may be required with urine samples.<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 80 pg\/ml<br \/>\nRange: 93.8 pg\/ml &#8211; 6000 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27282"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27282"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27282"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}