{"id":27294,"date":"2021-02-14T18:28:22","date_gmt":"2021-02-14T15:28:22","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-elafin-elisa-kit-skalp-96-tests\/"},"modified":"2021-02-14T23:34:15","modified_gmt":"2021-02-14T20:34:15","slug":"human-elafin-elisa-kit-skalp-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-elafin-elisa-kit-skalp-96-tests\/","title":{"rendered":"Human Elafin ELISA Kit (Skalp)- 96 Tests"},"content":{"rendered":"<p>Product name<br \/>\nHuman Elafin ELISA Kit (Skalp)<br \/>\nDetection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nCell culture supernatant, Serum, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n= 5 pg\/ml<br \/>\nRange<br \/>\n6.86 pg\/ml &#8211; 5000 pg\/ml<br \/>\nRecovery<br \/>\n> 100 %<\/p>\n<p>Sample specific recovery<br \/>\nSample type Average % Range<br \/>\nCell culture supernatant 111.8 102% &#8211; 121%<br \/>\nSerum 115.7 105% &#8211; 125%<br \/>\nPlasma 112.5 103% &#8211; 122%<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Human<br \/>\nProduct overview<br \/>\nAbcam\u2019s Elafin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Elafin in serum, plasma and cell culture supernatants.<\/p>\n<p>This assay employs an antibody specific for Human Elafin coated on a 96-well plate. Standards and samples are pipetted into the wells and Elafin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Elafin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Elafin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.<\/p>\n<p>Notes<br \/>\nOptimization may be required with urine samples.<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 5 pg\/ml<br \/>\nRange: 6.86 pg\/ml &#8211; 5000 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27294"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27294"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27294"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}