{"id":27298,"date":"2021-02-14T18:28:24","date_gmt":"2021-02-14T15:28:24","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-vegf-elisa-kit-96-tests\/"},"modified":"2021-02-14T23:34:18","modified_gmt":"2021-02-14T20:34:18","slug":"human-vegf-elisa-kit-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-vegf-elisa-kit-96-tests\/","title":{"rendered":"Human VEGF ELISA Kit- 96 Tests"},"content":{"rendered":"<p>Detection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nCell culture supernatant, Serum, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n< 10 pg\/ml\nRange\n8.23 pg\/ml - 6000 pg\/ml\nRecovery\n> 100 %<\/p>\n<p>Sample specific recovery<br \/>\nSample type Average % Range<br \/>\nCell culture supernatant 103.5 92% &#8211; 113%<br \/>\nSerum 104.4 92% &#8211; 115%<br \/>\nPlasma 105.7 93% &#8211; 114%<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Human<br \/>\nProduct overview<br \/>\nAbcam\u2019s VEGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF in serum, plasma and cell culture supernatants.<\/p>\n<p>This assay employs an antibody specific for Human VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.<\/p>\n<p>Notes<br \/>\nOptimization may be required with urine samples.<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 10 pg\/ml<br \/>\nRange: 8.23 pg\/ml &#8211; 6000 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27298"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27298"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27298"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}