{"id":27305,"date":"2021-02-14T18:28:38","date_gmt":"2021-02-14T15:28:38","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/mouse-cd40-elisa-kit-96-tests\/"},"modified":"2021-02-14T23:34:24","modified_gmt":"2021-02-14T20:34:24","slug":"mouse-cd40-elisa-kit-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/mouse-cd40-elisa-kit-96-tests\/","title":{"rendered":"Mouse CD40 ELISA Kit- 96 Tests"},"content":{"rendered":"<p>Detection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nCell culture supernatant, Serum, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n< 3 pg\/ml\nRange\n2.06 pg\/ml - 1500 pg\/ml\nRecovery\n86 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 84.23 74% - 93%\nSerum 77.26 71% - 87%\nPlasma 76.68 69% - 87%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Mouse\nProduct overview\nAbcam\u2019s CD40 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CD40 in serum, plasma and cell culture supernatants.\n\n\nThis assay employs an antibody specific for mouse CD40 coated on a 96- well plate. Standards and samples are pipetted into the wells and CD40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\n\nGet higher sensitivity in only 90 minutes with Mouse CD40 ELISA Kit (TNFRS5) (ab206307) from our SimpleStep ELISA\u00ae range.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 3 pg\/ml<br \/>\nRange: 2.06 pg\/ml &#8211; 1500 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Mouse<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27305"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27305"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27305"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}