{"id":27310,"date":"2021-02-14T18:28:41","date_gmt":"2021-02-14T15:28:41","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/mouse-eotaxin-1-elisa-kit-96-tests\/"},"modified":"2021-02-14T23:34:29","modified_gmt":"2021-02-14T20:34:29","slug":"mouse-eotaxin-1-elisa-kit-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/mouse-eotaxin-1-elisa-kit-96-tests\/","title":{"rendered":"Mouse Eotaxin-1 ELISA Kit- 96 Tests"},"content":{"rendered":"<p>Detection method<br \/>\nColorimetric<br \/>\nPrecision<br \/>\nIntra-assay<br \/>\nSample n Mean SD CV%<br \/>\nOverall    < 10%\nInter-assay\nSample n Mean SD CV%\nOverall    < 12%\nSample type\nCell culture supernatant, Serum, Plasma\nAssay type\nSandwich (quantitative)\nSensitivity\n< 1 pg\/ml\nRange\n1.02 pg\/ml - 100 pg\/ml\nRecovery\n94 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 95.25 83% - 104%\nSerum 94.95 83% - 104%\nPlasma 92.54 84% - 103%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Mouse\nProduct overview\nAbcam\u2019s Eotaxin-1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Eotaxin in serum, plasma and cell culture supernatants.\n\n\nThis assay employs an antibody specific for mouse Eotaxin-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Eotaxin-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Eotaxin-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Eotaxin-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 1 pg\/ml<br \/>\nRange: 1.02 pg\/ml &#8211; 100 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Mouse<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27310"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27310"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27310"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}