Detection method
\nColorimetric
\nSample type
\nCell culture supernatant, Serum, Plasma
\nAssay type
\nSandwich (quantitative)
\nSensitivity
\n< 0.3 ng\/ml\nRange\n0.274 ng\/ml - 200 ng\/ml\nRecovery\n78 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 74.84 67% - 81%\nSerum 74.56 67% - 82%\nPlasma 84.97 68% - 95%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Mouse\nProduct overview\nAbcam\u2019s CX3CL1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CX3CL1 in serum, plasma and cell culture supernatants.\n\nThis assay employs an antibody specific for Mouse CX3CL1 coated on a 96- well plate. Standards and samples are pipetted into the wells and CX3CL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CX3CL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CX3CL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"
Key features and details
\nSensitivity: 0.3 ng\/ml
\nRange: 0.274 ng\/ml – 200 ng\/ml
\nSample type: Cell culture supernatant, Plasma, Serum
\nDetection method: Colorimetric
\nAssay type: Sandwich (quantitative)
\nReacts with: Mouse<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27313"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27313"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27313"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}