{"id":27323,"date":"2021-02-14T18:28:49","date_gmt":"2021-02-14T15:28:49","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/mouse-igfbp5-elisa-kit-96-tests\/"},"modified":"2021-02-14T23:34:44","modified_gmt":"2021-02-14T20:34:44","slug":"mouse-igfbp5-elisa-kit-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/mouse-igfbp5-elisa-kit-96-tests\/","title":{"rendered":"Mouse IGFBP5 ELISA Kit- 96 Tests"},"content":{"rendered":"<p>Detection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nCell culture supernatant, Serum, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n< 5 pg\/ml\nRange\n4.12 pg\/ml - 3000 pg\/ml\nRecovery\n97 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 91.26 80% - 101%\nSerum 115.3 86% - 130%\nPlasma 95.11 83% - 102%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Mouse\nProduct overview\nAbcam\u2019s IGFBP5 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IGFBP5 in serum, plasma and cell culture supernatants.\n\n\nThis assay employs an antibody specific for mouse IGFBP5 coated on a 96- well plate. Standards and samples are pipetted into the wells and IGFBP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGFBP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\n\nGet better reproducibility in only 90 minutes with Mouse IGFBP5 ELISA Kit (ab208345) from our SimpleStep ELISA\u00ae range.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 5 pg\/ml<br \/>\nRange: 4.12 pg\/ml &#8211; 3000 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Mouse<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27323"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27323"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27323"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}