{"id":27324,"date":"2021-02-14T18:28:50","date_gmt":"2021-02-14T15:28:50","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/mouse-igf1-elisa-kit-96-tests\/"},"modified":"2021-02-14T23:34:45","modified_gmt":"2021-02-14T20:34:45","slug":"mouse-igf1-elisa-kit-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/mouse-igf1-elisa-kit-96-tests\/","title":{"rendered":"Mouse IGF1 ELISA Kit- 96 Tests"},"content":{"rendered":"<p>Detection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nCell culture supernatant, Serum, Plasma, Tissue Lysate<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n< 4 pg\/ml\nRange\n2.74 pg\/ml - 2000 pg\/ml\nRecovery\n98 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 86.4 72% - 99%\nSerum 101.2 85% - 117%\nPlasma 106.6 85% - 114%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Mouse\nProduct overview\nAbcam\u2019s IGF1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IGF1 in serum, plasma and cell culture supernatants.\n\nThis assay employs an antibody specific for mouse IGF1 coated on a 96- well plate. Standards and samples are pipetted into the wells and IGF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGF1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 4 pg\/ml<br \/>\nRange: 2.74 pg\/ml &#8211; 2000 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum, Tissue Lysate<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Mouse<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27324"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27324"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27324"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}