Product name
\nMouse IGF2 ELISA Kit
\nDetection method
\nColorimetric
\nSample type
\nCell culture supernatant
\nAssay type
\nSandwich (quantitative)
\nSensitivity
\n< 1.5 ng\/ml\nRange\n2.3 ng\/ml - 150 ng\/ml\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Mouse\nProduct overview\nAbcam\u2019s IGF2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IGF2 in cell culture supernatants.\n\nThis assay employs an antibody specific for mouse IGF2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGF2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\nPlease note that ab100696 has only been validated with cell culture supernatant samples and cannot be used with plasma samples. It is also not validated for serum samples.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"
Key features and details
\nSensitivity: 1.5 ng\/ml
\nRange: 2.3 ng\/ml – 150 ng\/ml
\nSample type: Cell culture supernatant
\nDetection method: Colorimetric
\nAssay type: Sandwich (quantitative)
\nReacts with: Mouse<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27325"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27325"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27325"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}