{"id":27332,"date":"2021-02-14T18:29:04","date_gmt":"2021-02-14T15:29:04","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/mouse-il-17b-elisa-kit-96-tests\/"},"modified":"2021-02-14T23:34:52","modified_gmt":"2021-02-14T20:34:52","slug":"mouse-il-17b-elisa-kit-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/mouse-il-17b-elisa-kit-96-tests\/","title":{"rendered":"Mouse IL-17B ELISA Kit- 96 Tests"},"content":{"rendered":"<p>Detection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nCell culture supernatant, Serum, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n< 12 pg\/ml\nRange\n16.46 pg\/ml - 12000 pg\/ml\nRecovery\n> 100 %<\/p>\n<p>Sample specific recovery<br \/>\nSample type Average % Range<br \/>\nCell culture supernatant 149.4 139% &#8211; 161%<br \/>\nSerum 118.9 104% &#8211; 128%<br \/>\nPlasma 129.3 92% &#8211; 155%<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Mouse<br \/>\nProduct overview<br \/>\nAbcam\u2019s IL-17B Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-17B in serum (mouse IL-17B concentration is pretty low in normal serum\/plasma, it may not be detected in this assay), plasma and cell culture supernatants.<\/p>\n<p>This assay employs an antibody specific for mouse IL-17B coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17B present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-17B antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17B bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 12 pg\/ml<br \/>\nRange: 16.46 pg\/ml &#8211; 12000 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Mouse<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27332"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27332"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27332"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}