Detection method
\nColorimetric
\nSample type
\nCell culture supernatant, Serum, Plasma
\nAssay type
\nSandwich (quantitative)
\nSensitivity
\n< 8 pg\/ml\nRange\n4.1 pg\/ml - 1000 pg\/ml\nRecovery\n87 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 94.58 86% - 104%\nSerum 88.58 83% - 99%\nPlasma 87.94 82% - 98%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Mouse\nProduct overview\nAbcam\u2019s MIP-1a Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP-1a in serum, plasma, and cell culture supernatants.\n\nThis assay employs an antibody specific for mouse MIP-1a coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP-1a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP-1a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP-1a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"
Key features and details
\nSensitivity: 8 pg\/ml
\nRange: 4.1 pg\/ml – 1000 pg\/ml
\nSample type: Cell culture supernatant, Plasma, Serum
\nDetection method: Colorimetric
\nAssay type: Sandwich (quantitative)
\nReacts with: Mouse<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27351"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27351"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27351"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}