{"id":27366,"date":"2021-02-14T18:29:32","date_gmt":"2021-02-14T15:29:32","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/mouse-sdf1-elisa-kit-96-tests\/"},"modified":"2021-02-14T23:35:34","modified_gmt":"2021-02-14T20:35:34","slug":"mouse-sdf1-elisa-kit-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/mouse-sdf1-elisa-kit-96-tests\/","title":{"rendered":"Mouse SDF1 ELISA Kit- 96 Tests"},"content":{"rendered":"<p>Detection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nCell culture supernatant, Serum, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n< 3 pg\/ml\nRange\n9.38 pg\/ml - 600 pg\/ml\nRecovery\n99 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 99.34 89% - 107%\nSerum 99 91% - 107%\nPlasma 100.36 90% - 107%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Mouse\nProduct overview\nMouse SDF1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab100741) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse SDF1 in serum, plasma and cell culture supernatants. (Mouse SDF1 concentration is pretty low in normal serum\/plasma, it may not be detected in this assay).\n\n\nThis assay employs an antibody specific for mouse SDF1 coated on a 96-well plate. Standards and samples are pipetted into the wells and SDF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse SDF1antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SDF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 3 pg\/ml<br \/>\nRange: 9.38 pg\/ml &#8211; 600 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Mouse<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27366"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27366"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27366"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}