Detection method
\nColorimetric
\nSample type
\nCell culture supernatant, Plasma, serum free media
\nAssay type
\nSandwich (quantitative)
\nSensitivity
\n< 60 pg\/ml\nRange\n93.75 pg\/ml - 6000 pg\/ml\nRecovery\n93 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 92.46 84% - 104%\nPlasma 93.82 83% - 102%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Mouse\nProduct overview\nAbcam\u2019s TNF alpha Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TNF alpha in plasma and cell culture supernatants.\n\n\nThis assay employs an antibody specific for Mouse TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\n\nMouse TNFa levels are extremely low, and as such we expect \u201cnormal\u201d levels to be undetectable when using our kit on plasma samples.\n\n\nWe have not been able to detect endogenous Mouse TNF alpha in normal serum with ab100747, only in serum spiked with Mouse TNF alpha.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"
Key features and details
\nSensitivity: 60 pg\/ml
\nRange: 93.75 pg\/ml – 6000 pg\/ml
\nSample type: Cell culture supernatant, Plasma, serum free media
\nDetection method: Colorimetric
\nAssay type: Sandwich (quantitative)
\nReacts with: Mouse<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27372"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27372"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27372"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}